Cytochrome C Oxidase Subunit-1(COX1) Gene in Tilapia Oreochromis Niloticus: Its Cloning and Characterization

Abumourad, Iman; Meselhy, Salah; Nie, Pin

doi:10.21608/maj.2011.2676

Cytochrome C Oxidase Subunit-1(COX1) Gene in Tilapia Oreochromis Niloticus: Its Cloning and Characterization

Document Type : Original Article

Authors

¹ Department of Hydrobiology, National Research Center

² Fish Health Department the WorldFish Center, Cairo, Egypt and

³ State Key Laboratory of Freashwater Ecology and Biotechnology and Laboratory of Fish Diseases, Institute of Hydrobiology, Chinese Academy of Sciences, Hubei Province, China.

10.21608/maj.2011.2676

Abstract

The current study aimed to identify cytochrome c oxidase subunit 1 (ONCOX1) in Tilapia (Oreochromis niloticus) immunized by formalin-killed Flavobacterium columnarae. Suppressive subtractive hybridization (SSH) was utilized to construct a cDNA library and semi-quantitative RT-PCR analysis used to examine Oreochromis niloticus cytochrome c oxidase subunit 1 (COX1) gene expression. COX1 cDNA is composed of 1139 bps with a 1107 bps open reading frame, the predicted gene product is 369 amino acids with molecular weight of 40.16 kDa. The amino acid sequence revealed high identity with subunit 1 from Oreochromis mossambicus. Compared to ß-actin, the semi-quantitative RT-PCR revealed that ONCOX1 expressed in tissues of stimulated fish as up-regulated gene suggesting that this member of COX genes is probably involved in the general immune response against the pathogenic bacteria.

Keywords